nested polymerase chain reaction Search Results


nested polymerase chain reaction  (Verlag GmbH)
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Verlag GmbH nested polymerase chain reaction
Nested Polymerase Chain Reaction, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nested polymerase chain reaction  (Feto Maternal and GenetYX Center)
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Nested Polymerase Chain Reaction, supplied by Feto Maternal and GenetYX Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nested reverse transcription polymerase chain reaction (rt-pcr) assay  (DaAn Gene)
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Nested Reverse Transcription Polymerase Chain Reaction (Rt Pcr) Assay, supplied by DaAn Gene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nested polymerase chain reaction  (Promega)
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Promega nested polymerase chain reaction
Nested Polymerase Chain Reaction, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multiplex nested polymerase chain reaction  (College of American Pathologists)
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Multiplex Nested Polymerase Chain Reaction, supplied by College of American Pathologists, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nested polymerase chain reaction (pcr)  (Alpha Biolaboratory)
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Alpha Biolaboratory nested polymerase chain reaction (pcr)
Nested Polymerase Chain Reaction (Pcr), supplied by Alpha Biolaboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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filmarray nested multiplex polymerase chain reaction method  (BioFire Defense)
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nested polymerase chain reaction (pcr  (Becton Dickinson)
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Becton Dickinson nested polymerase chain reaction (pcr
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Nested Polymerase Chain Reaction (Pcr, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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multiplex nested reverse transcription polymerase chain reaction  (Promega)
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Promega multiplex nested reverse transcription polymerase chain reaction
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Multiplex Nested Reverse Transcription Polymerase Chain Reaction, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nested polymerase chain reaction  (Funakoshi ltd)
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Funakoshi ltd nested polymerase chain reaction
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Nested Polymerase Chain Reaction, supplied by Funakoshi ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hiv detection in subgingival and salivary specimens by nested reverse transcription polymerase chain reaction (nrt-pcr)  (CollaGenex Pharmaceuticals)
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CollaGenex Pharmaceuticals hiv detection in subgingival and salivary specimens by nested reverse transcription polymerase chain reaction (nrt-pcr)
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Hiv Detection In Subgingival And Salivary Specimens By Nested Reverse Transcription Polymerase Chain Reaction (Nrt Pcr), supplied by CollaGenex Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hiv detection in subgingival and salivary specimens by nested reverse transcription polymerase chain reaction (nrt-pcr) - by Bioz Stars, 2026-10
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specific t. pallidum nested polymerase chain reaction (npcr) amplifying the tpp47 gene of t. pallidum  (National Reference Center for Legionella)
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National Reference Center for Legionella specific t. pallidum nested polymerase chain reaction (npcr) amplifying the tpp47 gene of t. pallidum
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Specific T. Pallidum Nested Polymerase Chain Reaction (Npcr) Amplifying The Tpp47 Gene Of T. Pallidum, supplied by National Reference Center for Legionella, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by PCR with total rat testis RNA and a rat pachytene spermatocyte cDNA library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.

Journal:

Article Title: Acroplaxome, an F-Actin-Keratin-containing Plate, Anchors the Acrosome to the Nucleus during Shaping of the Spermatid Head

doi: 10.1091/mbc.E03-04-0226

Figure Lengend Snippet: Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by PCR with total rat testis RNA and a rat pachytene spermatocyte cDNA library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.

Article Snippet: We started cloning of Sak57 cDNA from a rat testis λgt11 cDNA library (BD Biosciences Clontech, Palo Alto, CA) by using nested polymerase chain reaction (PCR) with primers gt11-5′ and R followed by gt11-5′ (GACTCCTGGAGCCCG) and T. Sequencing of an obtained fragment revealed near identity to the mRNA for human keratin 5 (K5).

Techniques: Expressing, Affinity Purification, cDNA Library Assay, Clone Assay, Purification, Immunohistochemical staining, Immunofluorescence, Negative Control, Western Blot